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Instead of installing
MUMMIE (or microMUMMIE) directly on your system, you can download a
virtual machine in which MUMMIE has already been installed. This
may be easier than installing MUMMIE directly, since MUMMIE depends on
a number of libraries which need to be installed. First, you need to install Oracle's VirtualBox software. Next, download this file: ubuntu.vdi (3.73 GB) Follow the instructions at VirtualBox for mounting ubuntu.vdi as a virtual machine. For the current version of Virtual Box, this is done by clicking "New", following the installation wizard up to the "Hard Drive" step, and selecting the option "Use an existing virtual hard drive file" (these instructions may be different for a different version of VirtualBox). Once you've got ubunto.vdi mounted, follow these steps: 1. Create a shared folder called "data" which contains PARalyzer outputs, mature miRNA sequences, and 2bit genome files (click "Settings", then "Shared Folders", then "add"). 2. Open the virtual machine in VirtualBox (click "Start") 3. Open a terminal window by clicking on the xterm icon at left 4. Execute this command: cd mummie/src 5. Run microMUMMIE.pl on your data, which should be visible via the shared folder "data". For example: microMUMMIE
mature-miRNAs.txt genome.2bit paralyzer.distribution.csv out.gff
posteriors:0/1 >& messages.txt Note: The virtual machine is configured for a single user named "George Bush" (userid "george") with password "password". The virtual hard drive is limited to a maximum size of 8 GB, but you can access an unlimited amount of data via the shared folder. |